Figure 1

Presentation of Art v 1 _25-36 by APC expressing HLA-DR1 or -DR4. A. HLA-DR expression on the EBV cell lines used as APC. B. Stimulation of TCL (n = 9) and TCC (n = 17) derived from 5 different subjects with an optimum concentration of Art v 1_25-36 peptide (3 μM). Box plots indicating the median and quartile ranges are shown. Background proliferations for DR1 or DR4 plus T cells ranged from 1,002-27,612 cpm and 2406-21054 cpm for TCL and from 1525-4264 cpm and 370-4270 cpm for TCC). C. T cell reactivity to different concentrations of Art v 1_25-36 presented by HLA-DR1 or -DR4 T cells were stimulated for 3 days with DR1- or DR4-expressing APC and proliferation was measured by ³H-thymidine uptake during the last 16 hours. Background proliferations were 2796/3056 cpm for Pat 2, 2247/1318 cpm for Pat 8.