Figure 2

Mapping of minimal T cell epitopes and critical amino acids within Art v 1 _25-36 presented by HLA-DR1 or -DR4. T cell reactivity was tested with truncated and single mutant Art v 1_25-36 –peptides. T cells were stimulated for 3 days with DR1- or DR4-expressing APC and proliferation was measured by ³H-thymidine uptake during the last 16 hours; (+) denotes >50% and (–) >70% reduction of proliferation induced by KCIEWEKAQHGA (dpm). Background proliferations for DR1 or DR4 plus T cells ranged from 1,002-27,612 cpm and 2406-21054 cpm for TCL and from 1525-4264 cpm and 370-4270 cpm for TCC.