Association of HLA-DR1 with the allergic response to the major mugwort pollen allergen: molecular background

BMC Immunology

Table 1 Binding of Art v 1-peptides and mono-substituted peptides of Art v 1 _25-36 **to HLA-DRB101:01 and HLA-DRB104:01**

Peptide	Amino Acid sequence	^aratio of IC₅₀ of Art v 1-peptides/HA_306-318
		HLA-DR1	HLA-DR4
HA_304-318	PKYVKQNTLKLAT	1	1
Art v _119-30	NKKCDKKCIEWE	>55032	>926
Art v 1_22-33	CDKKCIEWEKAQ	2016	756
Art v 1_26-36	KCIEWEKAQHGA	14	15
Ala-25	A CIEWEKAQHGA	9	21
Ala-26	KA IEWEKAGHGA	16	3
Ala-27	KCA EWEKAQHGA	2596	>926	p1
Ala-28	KCIA WEKAQHGA	6	11	p2
Ala-29	KCIEA EKAQHGA	69	14	p3
Ala-30	KCIEWA KAQHGA	6	28	p4
Ala-31	KCIEWEA AQHGA	17	17	p5
Gly-32	KCIEWEKG QHGA	63	149	p6
Ala-33	KCIEWEKAA HGA	11	31	p7
Ala-34	KCIEWEKAQA GA	8	17	p8
Ala-35	KCIEWEKAQHA A	5	5	p9
Gly-36	KCIEWEKAQHGG	74	242

^a Relative binding activity. Binding of peptides to HLA-DR1 and -DR4 molecules was assessed by competitive inhibition of the high affinity interaction of biotinylated-HA_306-318 with HLA-DR1 and -DR4 as described in Methods. IC50 values were converted into relative activities (ratio between IC50 value and IC50 of HA_306-318) to take into account the differences of sensitivity of the binding assays. The IC50 of HA_306-318 was 1.8 nM for HLA-DR1 and 11 nM for -DR4.

ISSN: 1471-2172