Figure 5

Simultaneous co-expression of TGFß and IL10 in CD4⁺CD25⁺IFNγ⁺Foxp3⁺and CD4⁺CD25⁺IFNγ⁺CD127^-iTreg . PBL of 3 healthy control individuals were stimulated for 16 h using PMA/Ionomycin. Simultaneous co-expression of TGFß and IL10 in CD4⁺CD25⁺IFNγ⁺Foxp3⁺ and CD4⁺CD25⁺IFNγ⁺CD127^- iTreg was determined using six-color flow cytometry. (a, b) 0.5% of CD4⁺CD25⁺IFNγ⁺Foxp3⁺ and 0.7% of CD4⁺CD25⁺IFNγ⁺CD127^- iTreg co-expressed the immunosuppressive cytokines TGFß as well as IL10 intracellularly, and 5% of CD4⁺CD25⁺IFNγ⁺Foxp3⁺ and 14% of CD4⁺CD25⁺IFNγ⁺CD127^- iTreg produced TGFß but not IL10 (all percentages with respect to total of CD4⁺ PBL in the cell culture). Data are given as mean±SD. Unstim = incubated in medium, stimulated = incubated in PMA/Ionomycin.