Figure 5
From: Characterization of functional mannose receptor in a continuous hybridoma cell line
Flow cytometric analysis of 43MRuptake of FITC-Man-BSA. 43MR cells (1 x 106) were seeded in 6-well tissue culture dishes and incubated with FITC-Man-BSA in the presence or absence of excess mannan (1 mg/ml) at 37°C. Cells were also incubated with FITC-BSA as a negative control. Following incubation, the cells were harvested and fixed in 2% paraformalin for FACS. Cells were also harvested for unstained control samples. The number of events acquired for each sample was 3 x 104. Data shown are the relative change in mean fluorescence intensity as compared to the control. Data are the mean +/− S.D. of at least two independent experiments.