Figure 6
From: Characterization of functional mannose receptor in a continuous hybridoma cell line
Phagocytic uptake and binding of pHrodo-labeled-S. aureus and GFP- Candida albicans. 43MR cells (1 x 106) were seeded in 6-well tissue culture dishes and incubated with either GFP-labeled Candida albicans or pHrodo Staphylococcus aureus for 30 minutes at 37°. Following incubation, the cells were washed twice in PBS and fixed in 2% paraformaldehyde. Following fixation, the cells were stained for MR using the previously described monoclonal antibody. Images were collected by confocal microscopy. N, nucleus. Arrows indicate internalized particles.