Figure 2

Decreased inflammatory infiltrate in the hearts of Ccr2^−/−mice injected with CAWS. (A) Immunohistochemical staining of macrophages using the marker ER-HR3 in Ccr2^+/+ mice and (B) quantification (pixels) of infiltration, at the level of the root of the aorta. (C) Absolute number of T cells (CD4⁺). (D) Representative FACS plots of neutrophils as defined by the expression of CD11b⁺ and Gr-1⁺ in Ccr2^+/+ and (E) Ccr2^−/− mice, with (F) absolute number of neutrophils. (G) FACS of iMo (CD11b⁺, Ly6C^hi, Ly6G^int) in Ccr2^+/+ mice and (H) Ccr2^−/− mice, with (I) absolute number of iMo. (J) Representative plots of dendritic cells (CD11b⁺, CD11c⁺, I-Ab⁺) in Ccr2^+/+ mice and (K) Ccr2^−/− mice. (L) Absolute number of dendritic cells. A-L represents data collected from the heart at the level of the aortic root after two cycles of CAWS. Each bar represents the mean ± SE from a representative experiment with 6–8 mice per group.