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Figure 3 | BMC Immunology

Figure 3

From: Adoptive transfer of IL-4Rα+ macrophages is sufficient to enhance eosinophilic inflammation in a mouse model of allergic lung inflammation

Figure 3

Phenotype of macrophages pre- and post- transfer. A. BMM were prepared from RAG2-/- and IL-4RαxRAG2-/- mice and were stained with anti-CD11b (solid line) or control antibody (dotted line) as indicated and analyzed by FACS. B. BMM were prepared from RAG2-/- (solid line) and IL-4RαxRAG2-/- (dotted line) mice and were stained with anti-IL-4Rα as indicated and analyzed by FACS. C. BMM were prepared from RAG2-/- (solid line) and IL-4RαxRAG2-/- (dotted line) mice and transferred to BALB/c IL-4RαxRAG2-/- mice by IP injection at 5 × 106 cells per mouse. Six weeks later spleens were harvested and stained with anti-CD11b and anti-IL-4Rα and analyzed by FACS. Live spleen cells were gated on expression of CD11b; the IL-4Rα staining histograms are shown. These results are representative of 3 independent experiments. D. BMM were prepared from RAG2-/- (IL-4Rα+/+) and IL-4RαxRAG2-/- (IL-4Rα-/-) mice and transferred to IL-4RαxRAG2-/- mice by IP injection at 5 x106 cells per mouse. Six weeks later spleens were harvested and single cell suspensions were prepared. In addition, single cell suspensions were prepared from untreated BALB/c mice. Pooled spleen cells were cultured in the presence or absence of IL-4 (10 ng/ml) for 30 min as indicated. Cell lysates were prepared and immunoprecipitated with anti-STAT6. The precipitates were analyzed by western blotting with anti-phosphotyrosine antibody. The membranes were stripped and re-probed with anti-STAT6.

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