Transfer of IL-4Rα+ BMM alters cytokine and chemokine levels in the bronchalveolar lavage fluid (BAL). BMM were prepared from IL-4Rα+/+ or IL-4Rα-/- mice as described above. BMM (5 × 106) were transfered by IP injection to IL-4RαxRAG2-/- mice on day -1. On day 0 all mice were injected with TH2 cells derived from D011.10 mice (1 × 107). The mice were immunized with OVA/alum on day 1 as indicated, followed by boost and challenge as described in Figure 2. The BAL fluid from individual mice (n = 3-4) was evaluated for cytokine and chemokine levels using Pierce Searchlight. The mean +/- SEM is shown. These results are representative of 3 independent experiments. Significant differences between groups was determined using the Student's T-test.