Figure 4

Genotyping and macroscopical examination. A Genotyping of the offspring derived from founder 58 using PCR amplification and Sty I-HF digest. Half of the 1052 bp PCR products from heterozygous animal are digested into 603 bp and 449 bp fragments, while the Rag1 PCR product from wild type offspring was digested completely; N: negative control. 58: PCR product from founder 58 as positive control. B Normal thymus of wild type animal of the strain LEW-Rag1 em1/Ztm-Ragl^{eml Ztm}. C Hypoplastic thymus in a Rag1 mutant rat. D Comparison of the thymus sizes of wild type and Rag1 mutant rats. E Comparison of thymus weigths, *** : P < 0.0001.