Figure 5

CD8 T cell subpopulation distribution in the blood and endocervix. (A) A diagram of Sallusto and Lanzavecchia’s classification scheme of CD8 T cells based on CD45RA and CCR7 expression and relative perforin expression of the different subpopulation, (B) distribution of the different CD8 T cell subpopulations in a representative C. trachomatis-negative and C. trachomatis-positive women (C-D) Distribution of different CD8 T cells in the blood (C), and in the endocervix (D) of 16 women (n=16). Using flow cytometric analyses, CD8 T cells were identified in a population of cells from PBMC and endocervical cells by gating for CD3⁺CD8⁺ cells. CD8 T cells were further characterized based on CD45RA and CCR7 expression. Naïve cells are CD45RA⁺ CCR7⁺, central memory (TCM) cells are CD45RA^-CCR7⁺, effector memory (T_EM) cells are CD45RA^- CCR7^-, and terminally differentiated effector memory (T_EMRA) cells are CD45RA⁺ CCR7^-. The majority of CD8 T cells in the endocervix are T_EM CD8 subtype.