Figure 3

Cytokine profile of splenic lymphocytes in response to peptide L1 and L2. (A) CD4⁺ T cells from mice immunized with rLpp20 were incubated with each peptide for 72 h and cytokines in the culture supernatants were measured by sandwich ELISA. Medium without any antigen was used as control. Results were expressed as means ± S.D. of triplicate wells from three independent experiments. Significant difference from control was indicated by *(p < 0.05). (B) CD4⁺ T cells from mice immunized with peptide L1 and L2 was isolated. Relative levels of IFN-γ and IL-4 were determined by real-time PCR and fold changes calculated using the delta delta Ct method. CD4+ T cells isolated from mice treated with PBS in IFA were served as negative controls. The values shown were means ± SD of three experiments. Significant difference from PBS control group was indicated by * (p < 0.05).