Figure 2

Tumor microenvironment induces a shift in CD11b⁺cell populations. A. Proportion of splenic CD11b⁺ cell population in C57BL/6 control animals and animals inoculated 14 days earlier with EL-4 lymphoma cells. B. Proportion of different CD11b⁺ cell populations in spleen and tumor from EL-4 inoculated C57BL/6 mice. C. RT-PCR analysis of S100A9 RNA expression in sorted cells from spleen and EL-4 tumors, as indicated. The threshold cycle number was determined and relative expression compared to the reference gene (β-actin) and the target gene, respectively. D. Western blot analysis of S100A9 expression on sorted cell populations from spleen and EL-4 tumors, as indicated. The Gray Value (Median) from Adobe Photoshop were used to determined the level of S100A9 and the reference gene (β-actin) expression. Statistical analysis was performed using Student’s t-test (*p < 0.05; **p < 0.01;***p < 0.001).