Figure 5

Effect of CD11b⁺cell populations on T cell proliferation in vitro . A. Indicated numbers of CD11b⁺ cells sorted from the peritoneal exudate cells (PEC) of normal control or EL-4 carrying C57BL/6 mice were co-cultured for 3 days with splenic CD4⁺ T cells and CD11c⁺ cells and proliferation induced with anti-CD3 and anti-CD28 antibodies. T cell proliferation was determined as described in Materials and Methods. B. CD11b⁺ cells sorted from peritoneal exudate cells (PEC) of normal control or granuloma-carrying BALB/c mice were co-cultured with splenic CD4⁺ T cells and CD11c⁺ cells isolated form BALB/c mice and proliferation determined as in A. Dashed red lines in A and B indicate unsuppressed T cell proliferation (in the absence of added CD11b⁺ cells). C. CD11b⁺ cell subpopulations (5x10⁴/culture), as indicated, sorted from spleens of EL-4 tumor inoculated C57BL/6 mice were co-cultured with splenic CD4⁺ T cells and CD11c⁺ cells as in A. D. CD11b⁺ cell subpopulations, as indicated, sorted from spleens of TMPD granuloma-carrying BALB/c mice were co-cultured with CD4⁺ T cells and CD11c⁺ cells isolated BALB/c mice, as described in C. Proliferation in control cultures without added CD11b⁺ cells were analysed in parallel. The data shows mean +/− SD of triplicate cultures. Statistical analysis was performed using two-way ANOVA (** p < 0.01; *** p < 0.001) in panels A and B, and Student t-test (*p < 0.05; **p < 0.01; ***p < 0.001) in panels C and D.