Figure 5

Effect of ATG therapy on naive and memory Tregs. NOD mice were treated with ATG or isotype IgG twice with a 3-day interval. At day 3 post treatment, the mice were sacrificed. The spleen cells were prepared and stained with CD4, CD25 CD62L and Foxp3 antibodies and examined by flow cytometry. CD62L+Foxp3+ and CD62L-Foxp3+ Tregs were analyzed by gating the CD4+CD25+ cell population as shown in Figure 5A. The summary of splenic CD62L+Tregs was shown in Figure 5B. ILN: inguinal lymph node; PLN: pancreatic lymph node. An additional experiment was performed with similar results.