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Figure 3 | BMC Immunology

Figure 3

From: Age-related accumulation of T cells with markers of relatively stronger autoreactivity leads to functional erosion of T cells

Figure 3

Age-dependent increase in IL-10 production. A) Total cellular RNA isolated from the spleens of young and old mice was used to obtain cDNAs which served as templates for the real time PCR using IL-10-specific primers. Shown are means and standard errors of IL-10 mRNA quantities relative to the 18S rRNA for triplicate reactions of each sample for three individual mice per group. B-C) Spleen cells from young or old mice (N = 3) were stimulated with plate-bound anti-CD3 antibody. Following 3 day culture, cells were stimulated with PMA/ionomycin and supernatants were tested for interferon-γ (B) or IL-10 (C) content by ELISA. No IL-10 protein was detected in unstimulated control cells (data not shown)

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