Figure 4

Reduced T cell responsiveness in aged mice. A) Anti-CD3-induced proliferation (means ± SD) of spleen cells from young and old WT mice. Shown are values from individual mice from a representative of four separate experiments. B) EAE was induced in 3 (closed symbols) or 16 (open symbols) months old female WT mice. Shown are mean clinical scores of groups of ten mice each. C) Spleen cells from young (8-12 weeks old) and old (12-24 months old) mice were stained with anti-CD4 (extracellular stain) and anti-FoxP3 (intracellular stain). Shown are mean (± SD) percentage of FoxP3⁺ cells within gated CD4⁺ populations (upper panel), and representative histograms for young (plain line) or old (bold line) mice.