Figure 7

Earlier onset of in vitro T-cell disfunction in MTB mice. A) Anti-CD3-induced proliferation (means ± SD) of spleen cells from 7 weeks (young) or 17 months old (old) WT or MTB mice. B) Spleen cells from 20 weeks old MTB and WT mice were cultured in the presence or absence of anti-CD3 antibody for 3 days. Following subsequent 24 hour stimulation with anti-CD3, cells were stained with anti-annexin V and propidium iodide. C) Proliferation to PMA/ionomycin of purified T cells from 15 months old MTB or WT mice in the presence of irradiated WT antigen presenting cells.