Table 1 Primers for steady-state mRNA and primary transcript
Locus | Primer name | Primer 1 | Primer 2 |
|---|---|---|---|
IL-4 | MP 588 | ACAGGAGAAGGGACGCCAT | GAAGCCCTACAGACGAGCTCA |
IL-13 | MP 590 | AGACCAGACTCCCCTGTGCA | TGGGTCCTGTAGATGGCATTG |
IFN-γ | MP 592 | GGATGCATTCATGAGTATTGC | CCTTTTCCGCTTCCTGAGG |
IL-5 | MP 779 | AGCACAGTGGTGAAAGAGACCTT | TCCAATGCATAGCTGGTGATTT |
IL-17A | MP 1018 | ATCAGGACGCGCAAACATG | GCAGCAACAGCATCAGAGACA |
IL-17 F | MP 1020 | ATTCCAGAACCGCTCCAGTTC | GGGTCTCGAGTGATGTTGTAATCC |
IL-10 | MP 1016 | GGCGCTGTCATCGATTTCTC | GCTCCACTGCCTTGCTCTTATTT |
IL-10 pri | MP 1082 | CCAATGGGTACTAACCAGATGCT | AATTCATTCATGGCCTTGTAGACA |
T-bet | MP 602 | CAACAACCCCTTTGCCAAAG | TCCCCCAAGCAGTTGACAGT |
GATA3 | MP 604 | AGAACCGGCCCCTTATCAA | AGTTCGCGCAGGATGTCC |
RORγT | MP 1564 | CTGTTTCGAGCCTTGGGCT | AAAGTCAAATATGGAGCTGATGAGC |
actin | MP 598 | AGAGGGAAATCGTGCGTGAC | CAATAGTGATGACCTGGCCGT |
TBP | MP 935 | CTTCGTGCAAGAAATGCTGAATAT | TGTCCGTGGCTCTCTTATTCTCA |