Figure 1
From: Proton channel HVCN1 is required for effector functions of mouse eosinophils
HVCN1 mRNA and protein expression levels in lung tissue and eosinophils. (A-B) The microarray (A) and real-time RT-PCR (B) analyses show increased Hvcn1 levels in the lung tissue of the allergen (Aspergillus)-challenged mice compared to saline-challenged controls. (C) Real-time RT-PCR analysis of Hvcn1 mRNA expression levels relative to housekeeping gene Actb (β-actin) in WT and HVCN1-deficient eosinophils and neutrophils from the different source (seen in “Methods”). Data are expressed as mean ± SD of 4–6 mice per group. **, P < 0.01. (D) Western blotting of HVCN1 in BM-derived eosinophils from WT and HVCN1-deficient mice, the representative of 4 experiments.