Figure 2

Bacterial uptake and phagocytosis by peritoneal macrophages in the control mice and mice that underwent laparotomy or laparoscopy. Bacterial uptake and phagocytosis were assessed after peritoneal macrophages were incubated with heat-killed E. coli or S. aureus for 15 (A) and 30 (B) min and compared between control (n = 14), laparotomy (n = 14), and laparoscopy (n = 14) groups. Data are mean ± SD of triplicate samples from at least five to seven independent experiments and expressed as mean channel fluorescence (MCF) per cell. *p < 0.05 compared with control mice, ^≠p < 0.05 compared with mice that underwent laparotomy.