Figure 7
From: The timing of TGF-β inhibition affects the generation of antigen-specific CD8+ T Cells
The effect of pretreatment with sTGF-βR on the generation of E7-specific CD8+T cells after immunization with Ad.E7. (A). Groups of mice (n=3) were treated with either IgG2a (control) or sTGF-βR 2 days before immunization with Ad.E7. Seven days after immunization, splenocytes from these animals were subjected to flow-cytometric analysis using anti-CD8 antibody and E7 tetramer. Displayed values are mean percentages of E7-tetramer positive CD8+ T cells among total splenic CD8+ T cells. Error bars represent SEM. The percentage of E7-specific CD8+ T cells in animals treated with sTGF-βR (“TGF-β-blockade”) was significantly less than the percentage of E7-specific CD8+ T cells in control animals treated with IgG2a (“Control IgG2a”): 0.6% and 1.9%, respectively (* = p < 0.01). (B). Examples of two-color (FITC and APC) flow cytometry dot plots in the analysis of CD8+ T cells isolated from the groups described in part A. Displayed values are percentages of CD8+ E7 tetramer-positive cells among total splenic CD8+ T cells. “Naïve” = non-immunized animals; “Control IgG2a/Ad.E7” = animals treated with IgG2a 2 days before immunization with Ad.E7. “TGF-β-blockade/Ad.E7” = animals treated with sTGF-βR 2 days sbefore immunization with Ad.E7.