Figure 1

CD1c-restricted T cells are reactive against the endogenous lipid phosphatidylcholine. HeLa cells were transfected with CD1c and CD1d constructs and used as antigen presenting cells. The HeLa-CD1 cells were loaded with 50 μg/ml phosphatidylcholine (PC) and used to stimulate CD1c-restricted T cells for 24 hours. Supernatants were collected and used in an IFN-γ ELISA. Data represent results from one of three independent experiments.