Figure 2

Increased cholesterol production does not alter CD1c/CD1d expression, but induces IFN-γ in CD1c-restricted T cells. (A) Jurkat cells were cultured in the presence of varying amounts of mevalonate, a precursor and inducer of cholesterol. CD1c and CD1d expression was assessed 10 days later via FACS. Data were averaged from three independent experiments. (B) Jurkat cells were either untreated or cultured with 200 μM mevalonate for 10 days and then used to stimulate CD1c-restricted T cells for 24 hours. Supernatants were collected and analyzed by IFN-γ ELISA. Standard deviation bars are averaged from three separate experiments.