Figure 3

Effects of HIV-1 on CD1c/CD1d expression. (A) HIV-1 infection modulates CD1c/CD1d expression. Jurkat cells were mock or HIV-1 IIIb infected for 10 days, and the levels of CD1c and CD1d expression were measured by FACS. CD1c/CD1d expression was normalized using uninfected, treated controls and plotted as % modulation from the controls. (B) Blocking cholesterol production results in significant CD1c/CD1d downregulation during HIV-1 infection. Jurkat cells were either treated with simvastatin, an inhibitor of cholesterol synthesis, or infected with HIV IIIb and cultured for 5 days. The levels of CD1c and CD1d expression were assessed by FACS, and data were normalized using uninfected, treated controls and plotted as % modulation from controls. All data represent results from one of three independent experiments.