Figure 4

Vpu, but not Nef, plays a role in CD1c/CD1d modulation. Jurkat cells were infected with wild-type (WT), delta Vpu (ΔVpu), or delta Nef (ΔNef) NL4-3 viruses for 2 days (A) or 10 days (B). The expression levels of CD1c and CD1d were then determioned by FACS and expressed as Mean Fluorescence Intensity (MFI). Data represent results from one of three independent experiments. (C) HIV-1 RNA copy number at each time point (2 days and 10 days post-infection) was determined by qPCR. HIV-1 RNA copy number was normalized based on the copy number of GAPDH. Data represent results from one of three independent experiments.