Increased TLR-4-associated adaptor molecule expression in human DCs matured with proTα or proTα(100–109). (A) Representative immunoblots of TRIF, TIRAP and MyD88 expression in cell extracts of iDCs and DCs matured with LPS, proTα or proTα(100–109) for 1 h and 18 h. GAPDH was used to verify equal loading. (B) Densitometry analysis of the adaptor proteins TRIF, TIRAP and MyD88, expressed as relative (%) expression versus GAPDH. Mean values of 4 independent experiments ± SD are shown. * p < 0.05; ** p < 0.01; *** p < 0.001.