Characterization of CSN1S1 differentiated monocytes, and the role of M-CSF. a. phagocytic uptake of labelled zymosan particles in an colorimetric assay of cells stimulated with 10 μg/ml CSN1S1 or medium control (mean and SD of 5 experiments). *, p < .05; **, p < .005, two-sided t-test. b. Representative flow cytometry data of monocytes differentiated towards a dendritic cell phenotype by GM-CSF, and reversal of differentiation by addition of CSN1S1 (representative data of 4 experiments). c. Stimulation of monocytes for 24 h with 10 μg/ml CSN1S1 in the presence and absence of an anti M-CSF antibody demonstrates neutralization of secreted M-CSF by the antibody (mean and SD of 4 experiments. *, p < .05, t-test). d. Effect of M-CSF neutralization by an M-CSF antibody on expression of CD14 and the receptors of M-CSF (CD115) or GM-CSF (CD116) (mean and SD of 4 experiments. *, p < .05, t-test).