Figure 3
Mtb-matured DCs show similar allogeneic and SEB-specific T cell priming capacities. DC were stimulated at day 5 for 24 h with the indicated reagents and then used to stimulate allogeneic T cells (a) or autologous T cells in the presence of SEB (b). After 3 days proliferation was assessed by [3H]-thymidine incorporation. a. The two experiments shown are representative of 3 independent experiments. Error bars represent the SEM of triplicate samples from one proliferation assay. b. The data of 4 experiments are shown. For experiment 1 the error bars represent the SEM of triplicate samples from one proliferation assay. For the experiments 2–4 data for the highest/optimal proliferation values of each experiment are shown. Error bars represent the SEM of triplicate samples of one proliferation assay. Note high variations between the different donors.