Figure 1

Reduction in lymphoid progenitor subsets in hoxa9−/− mice. A) Lymphoid light scatter gate (LLS) used throughout these studies. B) Flow cytometric analysis of BM Lin-IL-7R+c-kitlo CLP subsets in wildtype B6 and hoxa9−/− mice. The gating strategy is depicted from top to bottom with arrows. The lineage cocktail includes antibodies to CD45R/B220, CDllb/Mac1, Ly6G/Gr1, TER119, CD3ε, CD8α, CD11c, NK1.1, and Ly6C. Lin-IL-7R+ cells (top panel) were gated for IL-7R+c-kitlo cells (middle panel) and Lin-IL-7R+c-kitlo cells were fractionated into 3 subsets based on differential expression of Flt3 and Ly6D. ALPs are gated as Lin-IL-7R+c-kitloFlt3+Ly6D- and BLP as Lin-IL-7R+c-kitloFlt3+Ly6D+. A third Lin-IL-7R+c-kitloFlt3-Ly6D- subset is labeled as Flt3-Ly6D-. C) Summary of ALP, BLP, and Flt3-Ly6D- CLPs precursor frequencies in B6 (white bars) and hoxa9−/− (gray bars) mice. Precursor frequencies were calculated by multiplying sequential percentages of each gated region (ie., % Lin-IL-7R + in LLS gate x % ckitlo x %’s of ALP, BLP, or Flt3-Ly6D-). Data is representative of 5 individual B6 and hoxa9−/− animal BM analyses. The asterisk indicates p < 0.05. Error bars represent SEM.