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Figure 3 | BMC Immunology

Figure 3

From: Differential requirement for Hoxa9 in the development and differentiation of B, NK, and DC-lineage cells from Flt3+ multipotential progenitors

Figure 3

Hoxa9 is dispensable for the development of CDP in BM. A) Flow cytometry profile of CDP in B6 and hoxa9−/− mice. The Lin+ antibody cocktail included CD45R/B220, CDllb/Mac1, Ly6G/Gr1, TER-119, CD3ε, CD8α, and NK1.1. The gating strategy is depicted from top to bottom with arrows. CDP are identified as Lin-ckitloFlt3+ CD115/M-CSFR+. B) CDP precursor frequency. Precursor frequencies were calculated by multiplying sequential percentages of each gated region as described in Figure1. Data is representative of 4 individual mice per genotype. Error bars represent SEM.

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