Figure 1

Validation of cDNA array results using real time PCR in tissue lesions of CL patients. Relative mRNA levels of IFN-γ, IL-10, MCP-1, IL-1β, IRF-1 and Fas were determined by QPCR in tissues lesions of CL patients (n = 10) or control tissues (n = 7). The relative quantification of products was determined by the number of cycles over endogenous control (β-actin mRNA) required to detect the gene expression of interest. Values of P were calculated using unpaired Student’s t test. Horizontal lines indicate mean values.