Figure 1From: Engrafted human cells generate adaptive immune responses to Mycobacterium bovis BCG infection in humanized miceNSG-A2-BLT mice mount adaptive immune responses to BCG infection. NSG-A2-BLT mice were infected i.v. with BCG at a dose of 1 × 105 CFU per mouse. One month later mice were sacrificed, and T cell recall response of splenocytes was measured by ELISPOT (n = 6 for infected NSG-A2-BLT mice and n = 2 for uninfected NSG-A2-BLT mice) (A) and ELISA (n = 3 for infected NSG-BLT mice) (B). Culture conditions and antigenic stimulation are described in Methods. Abbreviations: CFP, Culture filtrate proteins; WC, Heat-killed whole BCG; PMA, Phorbol 12-myristate 13-acetate; Iono, ionomycin. *p < 0.05.Back to article page