Figure 10

Th1 and Th2 cytokine responses to stimulated myeloid DCs, demonstrating the effect of Der p 1 taken up via IgG 2C7, and using non-specific human IgG antibodies as controls, on naïve T cell polarisation. Myeloid DCs were pre-loaded with Der p 1 or deglycosylated Der p 1, both with and without IgG 2C7 antibody, prior to establishing DC-naïve T cell co-cultures. T cells were re-stimulated at day 10 with PMA (15 ng/ml) and Ionomycin (1ug/ml). The following conditions were used: cells only, Der p 1 or deglycosylated Der p 1, with and without IgG 2C7 (n = 4 and n = 5, respectively), and Der p 1 or deglycosylated Der p 1, with IgG controls (n =3). The results were statistically significant for IFN-γ when myeloid DCs were pre-loaded with Der p 1 and IgG 2C7 (*:p ≤ 0.05).