Th1 and Th2 cytokine responses to stimulated myeloid DCs, demonstrating the effect of Der p 1 taken up via IgE or IgG 2C7 uptake on naïve T cells polarisation. Myeloid DCs were pre-loaded with Der p 1 or deglycosylated Der p 1, both with and without IgE 2C7, prior to establishing DC-naïve T cell co-cultures. T cells were re-stimulated at day 10 with PMA (15 ng/ml) and Ionomycin (1ug/ml). The following conditions were used: cells only and Der p 1 or deglycosylated Der p 1, with and without IgE 2C7 (n = 3, except for deglycosylated Der p 1 only condition which was done twice). The results were statistically significant for IL-4 when myeloid DCs were pre-loaded with Der p 1 and IgE 2C7 (*:p ≤ 0.05).