Expression of surface molecules on resting SPM, BM and PM was determined by flow cytometry. Red solid lines, staining with (A) anti-CD115, anti-CD206, anti-Gr-1, (B) anti-CD80, anti-CD86, anti-MHC II, (C) anti-B7-H1, anti-B7-H2, anti-B7-H3 and anti-B7-H4; grey filled , staining with the relevant isotype controls. The percentage positivity is shown at the upper right of each histogram. Data are representative of 5 separate experiments of each macrophage preparation. D: summary data of surface molecules expression. Data are mean ± SEM. *p < 0.05, **p < 0.01.