Figure 3

Functional test assessing STAT3 phosphorylation. PBMCs were incubated with healthy donor (HD) or patients’ sera in a 1:10 dilution and subsequently stimulated with IL-10. Cells were lysed and examined for STAT3 phosphorylation by ELISA. An anti-IL10R2 antibody was used as a positive control. The OD of the healthy donor control was set as 100%.