Figure 1From: A PKC-SHP1 signaling axis desensitizes Fcγ receptor signaling by reducing the tyrosine phosphorylation of CBL and regulates FcγR mediated phagocytosisTime course analysis of tyrosine phosphorylation and dephosphorylation of CBL and its association with the CRKL adapter protein following FcγR1 activation. Anti-phosphotyrosine immunoblot was performed on CBL immunoprecipitated from U937 cells with anti-phosphotyrosine antibody (4G10). Lane 1, preimmune (1 mg/ml rabbit IgG) and lane 2, resting U937 cells (NS). FcγR1 stimulation [32.2mAb F(ab)2] and crosslinking with rabbit anti mouse antisera to U937 cells were done for 1, 5, 10, 20, 40 & 60 min., lane 3 to 8 respectively. Lane 9, whole cell lysate (WCL). This blot was again reprobed with anti-CBL and anti-CRKL antibody to confirm equal precipitation of CBL in all lanes and its association with CRKL. This experiment was repeated three times.Back to article page