Depigmented-polymerised house dust mite extract stimulates significantly reduced IL-17 production than unmodified native extract. PBMC (2×106/mL) were cultured with depigmented-polymerised (Depig-pol), depigmented (Depig) or native HDM extracts at different concentrations for different times: cytokine production was assessed by CBA, as described in Methods. N = 6. Statistical analysis by ANOVA. Dose-response for IL-10, IL-5, IFNγ and IL-17 production by Depig-pol, and native HDM extract-stimulated PBMC at day 5 (IL-10) or day 7 (IL-5, IFNγ and IL-17).