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Figure 3 | BMC Immunology

Figure 3

From: Immunobiotic Lactobacillus jensenii as immune-health promoting factor to improve growth performance and productivity in post-weaning pigs

Figure 3

Effect of Lactobacillus jensenii TL2937 on porcine intestinal epithelial (PIE) cells co-cultured with adherent population from swine Peyer’s Patches (PPs) under inflammatory conditions. PIE-Adherent cells co-cultures were treated with L. jensenii TL2937 or L. plantarum TL2966 for 48 h. Untreated PIE-adherent cells co-cultures were used as controls. After lactobacilli stimulation, treated cells and untreated controls were challenge with ETEC (5 × 107 cells/ml). (A) Expression of IL-1β, IL-8, IL-6, MCP-I, and TGF-β mRNAs was examined in PIE cells using RT-qPCR. (B) Expression of IL-1β, IL-6, IL-10, IFN-γ, and TGF-β mRNAs was examined in adherent cells using RT-qPCR. Values for bars with different letters were significantly different (P < 0.05). Values for bars with shared letters do not differ significantly. The results represent data from three independent experiments using ileal PPs from at least three different swine.

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