Figure 5

Effect of Lactobacillus jensenii TL2937 on negative regulators of the TLR signaling pathway in porcine intestinal epithelial (PIE) cells co-cultured with adherent population from swine Peyer’s Patches (PPs). PIE-Adherent cells co-cultures were treated with L. jensenii TL2937 or L. plantarum TL2966 for 48 h. Untreated PIE-adherent cells co-cultures were used as controls. Expression of SIGIRR, A20, Bcl-3, MKP-1, and IRAK-M mRNAs was measured in (A) PIE cells and (B) adherent cells using RT-qPCR. Values for bars with different letters were significantly different (P < 0.05). Values for bars with shared letters do not differ significantly. The results represent data from three independent experiments using ileal PPs from at least three different swine.