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Table 2 Tissue independent yield and QC on microarray

From: Validation and implementation of a method for microarray gene expression profiling of minor B-cell subpopulations in man

  Protocol Demographic data Array type
Tissue/B-cell subsets Sorted cells (x1000) Total no of Amp. samples Excluded Amp. yield cDNA μg Gender Age
PBMNC (F) n = 6 PreP     5 F; 1 M 49 ± 13 Exon
I 9-43 6 0 5.3 ± 0.9    
N 100-130 6 2 6.5 ± 0.9    
M 100-130 6 0 7.1 ± 0.6    
PB 4.5-29 6 1 5.3 ± 1.3    
PBMNC (F) n = 3 Lys     1 F; 2 M 43 ± 17 Exon
I 7.5 3 0 6.6 ± 1.0    
N 7.5 3 0 7.1 ± 1.1    
M 7.5 3 0 8.6 ± 1.5    
PB 4.5-7.5 3 0 9.3 ± 0.3    
PBMNC (C) n = 3 Lys     Identical   
I 7.5 3 0 6.0 ± 1.5    
N 7.5 3 0 7.3 ± 1.1    
M 7.5 3 0 6.4 ± 1.2    
PB 4.5-7.5 3 1 9.8 ± 0.5    
Tonsils (C) n = 8 Lys     6 F; 2 M 17 ± 10 U133
N 10-25 8 2 6.8 ± 0.8    
CB 10-20 8 1 8.8 ± 0.9    
CC 10-12.5 8 1 8.8 ± 0.9    
M 10-20 8 2 6.7 ± 0.9    
PB 5.6-20 8 1 9.3 ± 0.6    
Thymus (F) n = 7 Lys     2 F; 5 M 63 ± 12 Exon
N 5-25 6 2 7.6 ± 2.2    
M IgM 3.5-25 5 1 6.4 ± 3.1    
M IgG 10-33 7 2 8.4 ± 3.1    
PB 1-4.5 6 1 7.2 ± 4.1    
BM (F) n = 7 Lys     Identical   
PreBI 3-25 6 0 7.8 ± 2.2    
PreBII 3-25 7 1 7.8 ± 1.2    
I 0.8-25 7 2 6.0 ± 1.7    
N 8-25 7 0 6.3 ± 2.2    
M 2-25 7 1 7.1 ± 2.5    
PC 7.5-25 7 1 8.0 ± 2.2    
  1. The protocol was applied to PBMNC, tonsils, thymus and BM. The samples were sorted from either fresh (F) or cryopreserved (C) cells into PreProtect (PreP) or lysis/binding buffer (Lys). Excluded samples were either due to failed amplification or QC on array.