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Table 2 Tissue independent yield and QC on microarray

From: Validation and implementation of a method for microarray gene expression profiling of minor B-cell subpopulations in man

 

Protocol

Demographic data

Array type

Tissue/B-cell subsets

Sorted cells (x1000)

Total no of Amp. samples

Excluded

Amp. yield cDNA μg

Gender

Age

PBMNC (F) n = 6

PreP

   

5 F; 1 M

49 ± 13

Exon

I

9-43

6

0

5.3 ± 0.9

   

N

100-130

6

2

6.5 ± 0.9

   

M

100-130

6

0

7.1 ± 0.6

   

PB

4.5-29

6

1

5.3 ± 1.3

   

PBMNC (F) n = 3

Lys

   

1 F; 2 M

43 ± 17

Exon

I

7.5

3

0

6.6 ± 1.0

   

N

7.5

3

0

7.1 ± 1.1

   

M

7.5

3

0

8.6 ± 1.5

   

PB

4.5-7.5

3

0

9.3 ± 0.3

   

PBMNC (C) n = 3

Lys

   

Identical

  

I

7.5

3

0

6.0 ± 1.5

   

N

7.5

3

0

7.3 ± 1.1

   

M

7.5

3

0

6.4 ± 1.2

   

PB

4.5-7.5

3

1

9.8 ± 0.5

   

Tonsils (C) n = 8

Lys

   

6 F; 2 M

17 ± 10

U133

N

10-25

8

2

6.8 ± 0.8

   

CB

10-20

8

1

8.8 ± 0.9

   

CC

10-12.5

8

1

8.8 ± 0.9

   

M

10-20

8

2

6.7 ± 0.9

   

PB

5.6-20

8

1

9.3 ± 0.6

   

Thymus (F) n = 7

Lys

   

2 F; 5 M

63 ± 12

Exon

N

5-25

6

2

7.6 ± 2.2

   

M IgM

3.5-25

5

1

6.4 ± 3.1

   

M IgG

10-33

7

2

8.4 ± 3.1

   

PB

1-4.5

6

1

7.2 ± 4.1

   

BM (F) n = 7

Lys

   

Identical

  

PreBI

3-25

6

0

7.8 ± 2.2

   

PreBII

3-25

7

1

7.8 ± 1.2

   

I

0.8-25

7

2

6.0 ± 1.7

   

N

8-25

7

0

6.3 ± 2.2

   

M

2-25

7

1

7.1 ± 2.5

   

PC

7.5-25

7

1

8.0 ± 2.2

   
  1. The protocol was applied to PBMNC, tonsils, thymus and BM. The samples were sorted from either fresh (F) or cryopreserved (C) cells into PreProtect (PreP) or lysis/binding buffer (Lys). Excluded samples were either due to failed amplification or QC on array.
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BMC Immunology

ISSN: 1471-2172

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