Figure 1
CD8+T cells from VDR KO mice induce colitis in Rag KO recipients. Sorted CD8+ T cells from WT, IL-10 KO, VDR KO, and IL-10/VDR DKO mice were injected (106 cells per mouse i.p.) into Rag KO recipients. CTRL had no cell transfer. (A) Representative colonic sections from the Rag KO recipients of CD8+ T cells 8 weeks post-transfer. Colonic samples were stained with H&E (scoring system in Methods) and were shown at 10× magnification; scale bar = 50 μm. Colon sections shown were rated: WT (score = 0), IL-10 KO (score = 0), VDR KO (score = 7), and DKO (score = 8). (B) The percentage change in BW of Rag KO recipients of CD8+ T cells from WT, IL-10 KO, VDR KO and DKO mice. (C) The phenotype of CD8+ T cells from the spleen of WT and VDR KO mice. The splenic lymphocytes were prepared and stained for TCRβ, CD8β, CD44, CD62L, CD28 and CD122 antibodies. The gating strategy is in Additional file 2: Figure S2A. (D) The percentage change in BW of the Rag KO recipients following transfer of sorted CD8+/CD28+ or CD8+/CD28- cells from WT or VDR KO mice (106 cells per mouse i.p.). (E) Representative H&E stained colon sections from Rag KO recipients of CD8+/CD28+ or CD8+/CD28- T cells at 8 weeks post-transfer. Colon sections shown were rated: CTRL (score = 1), WT CD8/CD28+ (score = 3), WT CD8/CD28− (score = 3), VDR KO CD8/CD28+ (score = 6), VDR KO CD8/CD28− (score = 6). Values represent the mean ± SEM of 5-8 mice per group and one representative of three independent experiments (A-E). Two-way ANOVA (B,D) or Student’s t-tests (C), *P < 0.05, **P < 0.01.