Figure 2

VDR KO CD8⁺T cells aggravate CD4/CD45RB^highcell-induced colitis. Rag KO mice were injected i.p. with sorted 10⁶ WT or VDR KO (CD45.2⁺) CD8⁺ T cells on day -1 and 4 × 10⁵ WT (CD45.1⁺) CD4⁺CD45RB^high cells on day 0. (A) The percentage change in original BW of Rag KO mice recipients of CTRL, or CD4/CD45RB^high (CD4 only), CD4/CD45RB^high plus WT CD8 (CD4 + WTCD8), CD4/CD45RB^high plus VDR KO CD8 (CD4 + KOCD8) cells 7 weeks post-transfer. (B) The ratio of the colon/BW in the Rag KO recipients at week 7 post-transfer. (C) Representative sections of colonic tissue from CTRL (score = 0), CD4 only (score = 4), CD4 + WTCD8 (score = 6), and CD4 + KOCD8 (score = 6). Colonic samples were stained with H&E and are shown at 10× magnification; scale bar = 50 μm. (D) The isotype controls and intracellular staining for IFN-γ and IL-17A in CD8⁺ T cells in the IEL from Rag KO mice recipients of CD4 + WTCD8 or CD4 + KOCD8 T cells. (E) Total IFN-γ and IL-17A in cells from Rag KO recipients of CD4 + WTCD8 or CD4 + KOCD8 T cells. Grey histograms are isotype controls. Data is from n = 6-8 mice per group and the values represent the mean of three independent experiments ± SEM. ANOVA (A, B, D) and Student’s t-tests (E), *P <0.05, **P < 0.01, ***P < 0.001.