VDR KO CD8+T cells produce more and proliferate more rapidly to IL-2. (A) Histograms show CFSE dilution following in vitro proliferation of sorted WT and VDR KO CD8+ T cells in response to IL-2 stimulation or IL-2 stimulation with IL-2 neutralizing antibodies. The shaded histograms show the CFSE stained T cells prior to stimulation. (B) IL-2 production was measured from sorted CD8+ T cells with or without (unstim) CD3/CD28 stimulation. Supernatants were collected from 3d cultures of CD8+ T cells, and IL-2 in the supernatant was evaluated using ELISA. Values are the means from two independent experiments ± SEM. N.D., not detected. ANOVA (A) or Student’s t-test (B), *P < 0.05.