Figure 3

RA mediated suppression of T cell proliferation is not mediated by CD11c⁺BM-MCs. BM-MCs were magnetically separated with CD11c⁺ beads. Purity of CD11c⁺ and CD11c^- cells was confirmed and cells analyzed on the BD Accuri C6 Flow cytometer, Purified CD11c⁺(A) and CD11c^-(C) were co-cultured with responder immune cells for 96 hours with media or anti-CD3 stimulation and then pulsed with H³ thymidine in the final 18 hours of culture; and the relative percentages of CD11cs⁺(B) and CD11c^-(D) cells expressing maturation markers CD80, CD86 MHCII, PD-L1and PD-L2 were determined. Data are representative of three separate experiments* = p < 0.05.