Figure 3

rmIL-10 differentially modulated mRNA expression of Th subsets related cytokines and transcription factors in nasal mucosa. The mRNA relative expression levels of IL-5, IL-17, IL-10, TGF-β1, and TNF-α (C, E, G, H, and J) were significantly up-regulated in the AR group compared with the control group, and such up-regulation was markedly inhibited in AR mice with rmIL-10 treatment during challenge. Compared with the control group, IFN-γ mRNA expression levels (A) moderately increased without statistical significance,, but were significantly down-regulated with rmIL-10 treatment in AR mice. Compare with the control group, AR mice demonstrated significantly up-regulated the mRNA expression levels of GATA-3 and ROR-c in the nasal mucosa that were markedly suppressed with rmIL-10 treatment (D and F). Although AR mice had moderately increased Foxp3 mRNA expression levels compared with controls, but rmIL-10 treatment had no effect on Foxp3 mRNA expression (I). There was no significant difference for T-bet mRNA expression levels among different groups (B). T-bet, T-box transcription factor; GATA-3, GATA binding protein 3; ROR-c, retinoic acid-related orphan receptor C; Foxp3, Forkhead box P3. Control group, saline-sensitized and challenged mice; AR group, ovalbumin-sensitized and challenged mice; IL-10 group, AR mice were treated with rmIL-10 during challenge.