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Table III Secreted IFN-γ and TNF-α from T Cell Culture*

From: HIV infection and aging: enhanced Interferon- and Tumor Necrosis Factor-alpha production by the CD8+ CD28- T subset

Source Cells IFN-γ (pg/ml) TNF-α (pg/ml)
Aged PBMC 70,800 ± 8,500 (p < 0.001) 35,400 ± 6,200 (p < 0.005)
Control PBMC 20,300 ± 3,000 16,900 ± 4,100
Aged CD8+ 86,300 ± 15,600 (p < 0.001) 44,200 ± 7,800 (p < 0.005)
Control CD8+ 24,400 ± 6,200 19,400 ± 4,300
HIV+ CD8+ 121,000 ± 15,600 (p < 0.001) 57,200 11,500(p < 0.001)
Control CD8+ 27,300 ± 4,800 16,400 ± 4,900
  1. PBMC and purified CD8+ T cells were incubated for 66 hrs. as described in Methods, and the media was tested by ELISA. The results are expressed in pg IFN-γ or TNF-α per 105 cells (mean value ± S.D.). The standard deviation is shown and the p valves, determined with Student's t test, compare the IFN-γ or TNF-α secreted by aged or HIV+-derived cells and normal control cells. Eight different aged and HIV+ donors were used, and seven young control donors. In the presence of 5 mM of N-acetylcysteine, the production of both INF-γ and TNF-α was significantly increased in aged, HIV+ and control samples, generally by 1.5 to 2 fold.