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Figure 5 | BMC Immunology

Figure 5

From: Macrophages exposed continuously to lipopolysaccharide and other agonists that act via toll-like receptors exhibit a sustained and additive activation state

Figure 5

Combined effects of constitutively active toll-like receptors and added agonists. RAW264 cells were transiently co-transfected with the IL-12 promoter luciferase reporter genes and expression plasmids encoding wild-type and mutated (indicated by an asterisk) forms of the CD4/TLR chimaeric receptors as indicated either alone for TLR4 or in combination for TLR2 and 6. The experiment is described in detail in Materials and Methods. After overnight incubation, the cells were treated with either LPS [100 ng/ml], PAM3-CSK lipopeptide (100 ng/ml) or E-coli genomic DNA (10 μg/ml) as indicated. Each datapoint is the average of duplicate wells. The same pattern was observed in three separate experiments. The major variation between experiments was in the effect of the vector alone on basal reporter gene activity.

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BMC Immunology

ISSN: 1471-2172

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