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Figure 5 | BMC Immunology

Figure 5

From: Requirements for activation and RAFT localization of the T-lymphocyte kinase Rlk/Txk

Figure 5

Effect of Fyn on the half life of Rlk in the detergent soluble fraction. (A) 293T cells expressing WT Rlk with empty expression vector (top) or with Fyn WT (bottom) were pulse-labeled with Trans-35S-Label for fifteen minutes and chased with cold methionine and cysteine (20 mM each) for the indicated times. Cells were lysed at each time point, and the lysates were immunoprecipitated with anti-Rlk, washed, resolved with SDS-PAGE, and subject to fluorography. (B) Pulse-chase labeling of WT Rlk without (top) or with (bottom) kinase inactive Fyn.

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